| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048375 |
| Chromosome: | chromosome 1 |
| Location: | 6411879 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGTGGTGTGGGGAATGTGTGTGAGGAGATTTGTAAGGGGTTGCGGG |
| Internal bar code: | CGTACCCAATTATCTGGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 61.5385 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGTTTTTAAGTGCGGGC |
| Suggested primer 2: | ATGCCTGGAATACCCCTCCT |