Insertion junction: CLIP2.048376_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.048376
Chromosome:chromosome 7
Location:492824
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315700 FKB4,FKB16-1,FKB16A Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 1) PTHR10516//PTHR10516:SF250 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP16-1, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTGCCAGCTCCCGCCGTGAGCTGCTGACAGCCGTGGCACTGACCGCCG
Internal bar code:GGCGAATTTCAGGAATATTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:396
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCTTCACAAACTTGGGG
Suggested primer 2:ACTTGACACCTACCTTCGCG