| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048386 |
| Chromosome: | chromosome 9 |
| Location: | 4620149 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401663 | (1 of 1) K14795 - ribosomal RNA-processing protein 36 (RRP36) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCTAGGGGTCAAGGGTGGGGCTGTAGATACCAGTGCAAACTAAACC |
| Internal bar code: | GGGTACTATACGTCGTTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCCTTGAACTTGGGGTT |
| Suggested primer 2: | CACTCACACTCAATCGCCCT |