| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048398 |
| Chromosome: | chromosome 4 |
| Location: | 1648749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212700 | (1 of 19) PTHR31251//PTHR31251:SF1 - FAMILY NOT NAMED // SQUAMOSA PROMOTER-BINDING-LIKE PROTEIN 10-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTCTCCGACAAGCGCTGCTGCTCGGCGCCGATGTTAACAGCGCCGAT |
| Internal bar code: | AACCTGAATCTCGTGAACCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1469 |
| LEAP-Seq percent confirming: | 2.63158 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 111 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGGCCTGCTGTCAATAA |
| Suggested primer 2: | CCGCAAAACACTAACGCACA |