| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048447 |
| Chromosome: | chromosome 4 |
| Location: | 3420983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g800491 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAACCTAGACCACCTTTTGACCCTCCAGCGCAACCCCCCTGCCATTCC |
| Internal bar code: | AATGGCAATGTGCTAGAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2619 |
| LEAP-Seq percent confirming: | 68.4615 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGTCACCTGTTGTCCAG |
| Suggested primer 2: | CTGCTATTGTGCGCGTGTAC |