| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048453 |
| Chromosome: | chromosome 10 |
| Location: | 1232065 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426350 | predicted protein; (1 of 2) 5.2.1.14 - Beta-carotene isomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCATCCACCCACCACCAGTCAACCACCCTCTTGCTGCTGGCTGCGTGC |
| Internal bar code: | CTGGTAGTAGTCCACAAGCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1375 |
| LEAP-Seq percent confirming: | 89.7436 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCAGCGCGATGTTAAG |
| Suggested primer 2: | TCTCCACGTCGTTGACCTTG |