Insertion junction: CLIP2.048458_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048458
Chromosome:chromosome 8
Location:1917304
Confidence (%):63
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGAGCCTAGTTGGCCACCGCCACAACAAAGCAAACTATCAATTCGAGT
Internal bar code:TAGACGGGGTGATTGAGTTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCACAAACTTGCCAGCATCA
Suggested primer 2:TACACGCACGCATTGTTGTG