| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048461 |
| Chromosome: | chromosome 13 |
| Location: | 2425035 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579450 | CST1 | Chlamydomonas-specific membrane transporter of unknown function | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACCTTACTCTAAACACTCTACGCGCTAACTTAGCCTTCTGTCGTCGT |
| Internal bar code: | TAGTTCCTCGCCTATGTTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2155 |
| LEAP-Seq percent confirming: | 90.7895 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTGCAGAAAACCAGGGC |
| Suggested primer 2: | GCTGCTTCAACAGAAACGCA |