| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048464 |
| Chromosome: | chromosome 14 |
| Location: | 500071 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611200 | (1 of 10) PF02536 - mTERF (mTERF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGCACACGCGCCACCTCGGCCTGAGGGAGCCGGCATGCATGGAGTGA |
| Internal bar code: | GGGTAAAAGAAGGTGGAGACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2446 |
| LEAP-Seq percent confirming: | 70.9302 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCCATGTCAGTGACACG |
| Suggested primer 2: | CTTTGGTATCGCAATGCCGG |