| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048488 |
| Chromosome: | chromosome 1 |
| Location: | 5232396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036650 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTTCGCGCAGGTGGGTGGACGTGCGCATGGGAGCTGCAGCACAGGGT |
| Internal bar code: | TATTAATTAATGGTTGCATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4784 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCATCGTTCCAAATCTC |
| Suggested primer 2: | TGGGTATGGAGGACCTGGAG |