| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048494 |
| Chromosome: | chromosome 17 |
| Location: | 2903550 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802059 | (1 of 3) PF00078//PF00665 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Integrase core domain (rve) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTTGACCGGCTAACGAAAATGGTGCATCTTGTCCCTACGCGAGGCAC |
| Internal bar code: | TGGGATTGGAATCCACTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2618 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCATGCAACAGGTCCAGG |
| Suggested primer 2: | TCCGTGGTTCTCTAGCGAGA |