| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048515 |
| Chromosome: | chromosome 1 |
| Location: | 6038731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042650 | (1 of 17) PF13202 - EF hand (EF-hand_5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATCCTTCGACGCCGTGTCTCCTAGCATGTGCTGCCACAACCCTTCCA |
| Internal bar code: | TAGTAGATAGATTGAGTTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4259 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGATCCCGCAATGCTTTCT |
| Suggested primer 2: | CGCCTGCAATCATAACCACG |