| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048519 |
| Chromosome: | chromosome 12 |
| Location: | 9240983 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542800 | RPC2 | DNA-directed RNA polymerase III, subunit 2; (1 of 1) K03021 - DNA-directed RNA polymerase III subunit RPC2 (RPC2, POLR3B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCGTAGCGCCCGCACCTTGAGGAACGCAGGCAGCAGCGCGAACTTGT |
| Internal bar code: | TTAGTACAGTAGTGCTCTGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1314 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCATGGAGAAGCAAGACT |
| Suggested primer 2: | GTGTGTGTCTGCGTAGGTCA |