| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048526 |
| Chromosome: | chromosome 9 |
| Location: | 5395636 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408250 | EEF1A2 | Eukaryotic translation elongation factor 1-alpha; (1 of 1) PTHR23115//PTHR23115:SF170 - TRANSLATION FACTOR // ELONGATION FACTOR 1-ALPHA 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTCTGCCACTGCGCCGTCCTCCGCCTGCCACTCTTTATCCATTCCCCA |
| Internal bar code: | GTTTTGGATACCGGCTGTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 18.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGTACACACACACACAC |
| Suggested primer 2: | TTGTCTTCCTTCACTCCGGC |