Insertion junction: CLIP2.048539_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048539
Chromosome:chromosome 13
Location:2614506
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g580950 GT90-25,GT90F25 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 25 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAGCATCGATCTGTCGTGTGTGGGGCATGAGAGGTAGGCAGGAGTTGAG
Internal bar code:GGCATGTTATTTGCATCGTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2874
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCCCGCTACTCTAACCT
Suggested primer 2:AAAGCGCAAGTTGCATTCGT