| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048540 |
| Chromosome: | chromosome 12 |
| Location: | 9156199 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGTTTGCTCACACAGCTTGAGCAGCCTCCTGTTGTCATCGACACAGT |
| Internal bar code: | ACGCACACGCATAATATCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1296 |
| LEAP-Seq percent confirming: | 15.5172 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTGGCTTGCGATTACCG |
| Suggested primer 2: | CTGATTCGCGAGTGCTTTCG |