| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048545 |
| Chromosome: | chromosome 9 |
| Location: | 5032753 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405850 | NUO7 | NADH:ubiquinone oxidoreductase 49 kDa ND7 subunit; (1 of 1) K03935 - NADH dehydrogenase (ubiquinone) Fe-S protein 2 (NDUFS2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCGGCCGTGTGTCCGCCCCGCTGCCCTATCCCTGCGCAGACCCTCACA |
| Internal bar code: | ATGCGATTTGTGTAGTATCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1424 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCAATCAGCCCTTACA |
| Suggested primer 2: | AACCAGGCTGGAGCTTCTTC |