| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048551 |
| Chromosome: | chromosome 2 |
| Location: | 4707650 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108100 | (1 of 1) PTHR12147//PTHR12147:SF22 - FXNA-RELATED // ENDOPLASMIC RETICULUM METALLOPEPTIDASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGCGTCAGGTGTGTGGGCGGCAAGGCGCGACCAAAAAGCGGCGGTTTG |
| Internal bar code: | CTTGAGGCAGTATAATGATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2975 |
| LEAP-Seq percent confirming: | 98.7952 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCAAGCCGAGTGAAGC |
| Suggested primer 2: | GAACCATTCACCCAGCTCCA |