| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048553 |
| Chromosome: | chromosome 10 |
| Location: | 815731 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423200 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACGCAGGCACGGTGGGGGCATGTCACGGCCCAGCACACGAGCAATAC |
| Internal bar code: | CCCGCCGGGAGTTCGCAGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6504 |
| LEAP-Seq percent confirming: | 98.5816 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 141 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGCGGACTCAAGTGGTT |
| Suggested primer 2: | CATCTACCCACAAGAGCGCA |