| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.048555 |
| Chromosome: | chromosome 7 |
| Location: | 5543297 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351700 | (1 of 1) PTHR19315:SF9 - ER MEMBRANE PROTEIN COMPLEX SUBUNIT 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGTACGGCAGATGCACGATTAGCCCTTTACAATACACGTAAGGGACA |
| Internal bar code: | TAGGTATATAACGTCGCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1154 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGCTCGACACCAGTCC |
| Suggested primer 2: | ATACCCGACCGGAAGTCTCA |