| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048557 |
| Chromosome: | chromosome 3 |
| Location: | 601067 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145427 | (1 of 3) 2.1.1.100 - Protein-S-isoprenylcysteine O-methyltransferase / Prenylcysteine carboxyl methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACAGCAGCGCCAGGATGGCCAGGCGCGTCTCGTTGCGCGTGAGGATGG |
| Internal bar code: | GCCGTGCAACTAAGTAACAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3618 |
| LEAP-Seq percent confirming: | 81.5789 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCACAAATAGGGAGCCTT |
| Suggested primer 2: | AGCCGTACATGACCATAGCG |