Insertion junction: CLIP2.048572_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048572
Chromosome:chromosome 14
Location:2768029
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g626400 CYP33,CYP743C1 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGTTCGGATTTCGGTCTGTGGGTTGAGGCGCTGTGGCTGGTCACGGC
Internal bar code:ACCGTAGTGGCACTGGTAGTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2475
LEAP-Seq percent confirming:91.1111
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTCGGATCGAGGTTCGG
Suggested primer 2:ACACAGACTCGCAGACACAG