| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.048576 |
| Chromosome: | chromosome 2 |
| Location: | 7386156 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391986 | (1 of 1) 3.1.1.32 - Phospholipase A(1) / Phospholipase A1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTATCCAACTCCTACGCGCGCCCCGCAGACGCTGGCTGGGATTACA |
| Internal bar code: | TCGAGGATTCAGTATACTACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGTCGATGAGGCTGGT |
| Suggested primer 2: | CGCGAGTATAAGAGCCGGTT |