Insertion junction: CLIP2.048577_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048577
Chromosome:chromosome 6
Location:7371180
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g300001 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCCCAGCGGCACCAGCAGTAGCGGCAGTAGCAGTGCTGCCACGGACG
Internal bar code:GACGAACCAAGTCCATTCGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2571
LEAP-Seq percent confirming:88.0952
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CACTACTGTCACCACCCACC
Suggested primer 2:GAGGTGCGTGTGTGAGGTTA