Insertion junction: CLIP2.048584_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048584
Chromosome:chromosome 16
Location:5999124
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g678551 CYG34 (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTATGGACCGCCCGCCACCTGCAGCCTGGCTGCTGCGCCTGCCGAAC
Internal bar code:AAGGTGAGGAGCCAGTAAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3595
LEAP-Seq percent confirming:60.8696
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TACACCACCGCACTGCTTAG
Suggested primer 2:TTCAAAGGCTTCGTTGCTGC