Insertion junction: CLIP2.048591_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.048591
Chromosome:chromosome 7
Location:4661708
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344800 TNP21,CSB33 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCTCAAAAAAGGGGTTCCTCCTGTAAAAAAAGCATTATATTGGGTAC
Internal bar code:TAACAGAATTCATGGGGTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1925
LEAP-Seq percent confirming:96.9697
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:TGATGCGGGATTATGTGCGA
Suggested primer 2:ACAACTCCTCATCCAGCTGC