| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048593 |
| Chromosome: | chromosome 8 |
| Location: | 3495621 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378900 | ND3,NUO3 | (1 of 1) K03880 - NADH-ubiquinone oxidoreductase chain 3 (ND3); NADH:ubiquinone oxidoreductase ND3 subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGTGGTTGATGTCGGGGGCTGCGTTGGGCGCGGGGCTTGCGCTTTG |
| Internal bar code: | GCTATCGCTCCACTCTTTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTCTTGTCCAGGTCCAC |
| Suggested primer 2: | GACCACCAACACAACAAGGC |