| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048612 |
| Chromosome: | chromosome 2 |
| Location: | 1885589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087551 | (1 of 1) K17600 - vacuolar protein sorting-associated protein 54 (VPS54) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATCCCCCCTCCCCCGCAGTCCGCGGTGGCACTGGGCATGACGCCGCCGC |
| Internal bar code: | TTCGAGGATCGTCTCGGTCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1795 |
| LEAP-Seq percent confirming: | 51.8519 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTGCTAATGACCAGCA |
| Suggested primer 2: | GGGGGTCTTACCTGCAGTTC |