Insertion junction: CLIP2.048637_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048637
Chromosome:chromosome 16
Location:6705950
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g673150 HDA14 (1 of 1) PTHR10625:SF124 - HISTONE DEACETYLASE 9; RPD3/HDA1 type histone deacetylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCACCCCGGGCGCGTGCTCCAGCAGCCGCAGGTTCTCAAACACCTCCCG
Internal bar code:AGAGACTAAGCAGGTGGGTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1285
LEAP-Seq percent confirming:97.6744
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGAGGTTGTCGCAGAGTG
Suggested primer 2:CGACCTCGACAATACAGGGG