Insertion junction: CLIP2.048662_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.048662
Chromosome:chromosome 10
Location:5883011
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g460650 CYN52 (1 of 2) PTHR11071//PTHR11071:SF297 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE // CYCLOPHILIN-LIKE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FAMILY PROTEIN; Cyclophilin 52 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACTGGCCCCCCAGCGGCGACTCCACCCAGGCACCAGCCTGGTCGATGA
Internal bar code:ACCAACCTTTGGCCGGTACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2038
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAAACCGGTACACACACC
Suggested primer 2:ACCTCTACCCCATTCTCCCC