| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048681 |
| Chromosome: | chromosome 2 |
| Location: | 2805728 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094350 | (1 of 1) PTHR23316//PTHR23316:SF30 - IMPORTIN ALPHA // SUBFAMILY NOT NAMED; Importin Alpha 1b | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGAGGGTCAGGCGTGAGTATGAGAGACTGCATGTGGCACGTCAGCGA |
| Internal bar code: | TCGAAGTGCGGGACCTGAATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 758 |
| LEAP-Seq percent confirming: | 45.4545 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATGCCAAAGCTTCGCAA |
| Suggested primer 2: | CTAGCGGCATCTTGGAGGAG |