Insertion junction: CLIP2.048683_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048683
Chromosome:chromosome 3
Location:4519067
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g175926 5'UTR
Cre03.g800333 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACCCTATTATGAGTGTTACCTGGATAAGCTACAAACGGATATCCGGGG
Internal bar code:AGGCTCTCCAGGCTACAAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1984
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCCAGCCCTGATCCG
Suggested primer 2:GAGGCAAACCAGGAGCAGG