| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048687 |
| Chromosome: | chromosome 10 |
| Location: | 1447331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g428150 | (1 of 1) PTHR10566:SF58 - PROTEIN D2023.6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACCTGCAGCAAACACACCGCGGCATAAACACACAGTTGTTACGACGT |
| Internal bar code: | AATATGCGGATTGTTGGAACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2794 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAAGACCAACGACTGCCT |
| Suggested primer 2: | AGTTTCCCCTGTGCTCGATG |