Insertion junction: CLIP2.048694_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.048694
Chromosome:chromosome 1
Location:7691038
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g054050 (1 of 1) PF09834 - Predicted membrane protein (DUF2061) (DUF2061) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCGATTAACTGCCCATAACGCGACCTGGCGCACTGCTGCATTAGCA
Internal bar code:TTTCCGACTTTTTTCTTTATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1284
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTCCGCCCTTAGTCTACA
Suggested primer 2:CTCCGTACTCTGATCGGTGC