| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048700 |
| Chromosome: | chromosome 5 |
| Location: | 1994502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g236350 | (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCCCGACCTCCTGTGCCCACGGCTGCCGGGCGCCACCCCCTCCATCCC |
| Internal bar code: | TTCAGAGTTAGGTGAACAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1979 |
| LEAP-Seq percent confirming: | 79.2208 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTTCGGTCCAGCCTTC |
| Suggested primer 2: | CGTACAGGTCACAGTGCTGT |