Insertion junction: CLIP2.048704_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.048704
Chromosome:chromosome 17
Location:315053
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g698100 DeSI-3,DESI3 (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCACATGCTGCATTTCGGGCTGCGCGCTCCCGCCCCGCCACCAGGCGC
Internal bar code:GAGATTTATTAAATTTTGTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:371
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ATGGGCAACGACTACAAGGG
Suggested primer 2:GCTGTCCCATGATGTGTCCA