Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.048716 |
Chromosome: | chromosome 16 |
Location: | 718060 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g691150 | (1 of 11) PF13360 - PQQ-like domain (PQQ_2) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCATCCGTCGTGTTGCGTGTGTTGGAGGCCGAGGGCCCCCGGAACCGC |
Internal bar code: | AACGAGACTCATAAAGATTTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1683 |
LEAP-Seq percent confirming: | 82.3529 |
LEAP-Seq n confirming: | 70 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGGAAGCCCAGCTGATCA |
Suggested primer 2: | GCCTGTACTGGTCACCTTCC |