| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.048717 |
| Chromosome: | chromosome 6 |
| Location: | 2353734 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267850 | PLS1 | Phospholipid scramblase; (1 of 1) IPR005552//IPR025659 - Scramblase // Tubby C-terminal-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGTCCAACCTTACCCACGACAACGCGGCAAACTTGGAAGCCCTCTCG |
| Internal bar code: | TGCAGATCGAATTGGAATCCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 27.2727 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAATCGAAGCCCTCATCA |
| Suggested primer 2: | GGAGATATTCACGGACGCGT |