Insertion junction: CLIP2.048733_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048733
Chromosome:chromosome 2
Location:5275727
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112701 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAGACGACGCTGTCACCAGCGACTGCACCGGGCTAGCTGCCTCATGC
Internal bar code:GCGATGCGACATGCTATACTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1690
LEAP-Seq percent confirming:33.7838
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCATCCTGGGTTTCATCA
Suggested primer 2:GGGGAAGTGAGATAGGCAGC