Insertion junction: CLIP2.048737_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.048737
Chromosome:chromosome 7
Location:4821608
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346000 (1 of 22) PTHR11017//PTHR11017:SF145 - LEUCINE-RICH REPEAT-CONTAINING PROTEIN // SUBFAMILY NOT NAMED CDS/intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCATCCTTATCTCTTTGGACTGGCCGCAAGAGCTGCTCGACACAGAGCT
Internal bar code:ACAAGTAAAGAGGTAATGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1061
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACATCTGCCAGGTGCTC
Suggested primer 2:GTTTATGTCGTGCGGCTGTG