Insertion junction: CLIP2.048738_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048738
Chromosome:chromosome 6
Location:8432649
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308300 RRM6 (1 of 2) IPR002877//IPR029063 - Ribosomal RNA methyltransferase FtsJ domain // S-adenosyl-L-methionine-dependent methyltransferase; Putative ribosomal RNA methyltransferase of the RrmJ/FtsJ family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGAACTGTCAAAAACAACCCTCACCTCCTCCTCGTTTGCCCCTCACC
Internal bar code:AAGGTGAAGACACCTACCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:98
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCCCTATAGAACCCCCA
Suggested primer 2:CATCGGTCTTACGCTCCCTC