| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048813 |
| Chromosome: | chromosome 2 |
| Location: | 359367 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075500 | 5'UTR | ||
| Cre02.g800161 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGATAAAACGTAGGAGTCTGCGCGTATTCGAAGTGCCATGTTGCTGGC |
| Internal bar code: | CTTGTACTCTGTTAGTGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5287 |
| LEAP-Seq percent confirming: | 92.6829 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTTCAGTTTGCGCAAGAA |
| Suggested primer 2: | TTCCTTCACCAGAGCTGCTG |