| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048817 |
| Chromosome: | chromosome 7 |
| Location: | 2644514 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330500 | (1 of 5) PF04232 - Stage V sporulation protein S (SpoVS) (SpoVS) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTGGTTTTCAGTCCGGCTCGTTCTGGAGCCGGAGGGTGTTCGTCCGTA |
| Internal bar code: | AGCGAGCGATACTGAAATAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATGAACGGCACCAAAG |
| Suggested primer 2: | GGAGGATTGGCTGCCTTTCT |