| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048817 |
| Chromosome: | chromosome 11 |
| Location: | 1273424 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467696 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCACCCTCTTCCTCCCTGCGGGACGTCCACATGACAGCCCAAAAGGA |
| Internal bar code: | CGGGGTCTCGCTAATTGTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1441 |
| LEAP-Seq percent confirming: | 33.871 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAATGCTGTAGCTCGCCG |
| Suggested primer 2: | GCTTGACAAGGGAAGGAGCT |