Insertion junction: CLIP2.048824_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048824
Chromosome:chromosome 2
Location:8081892
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144350 RJL1 Small Rab-related GTPase; (1 of 1) K19372 - DnaJ homolog subfamily C member 27 (DNAJC27) intron
Cre02.g144400 uS12m,MRPS12 (1 of 1) K02950 - small subunit ribosomal protein S12 (RP-S12, MRPS12, rpsL); Mitochondrial ribosomal protein S12 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAAACCAAAGCCACCACCACCAAAAGCGCTGCCACCGCGGCCCATCCG
Internal bar code:GTCAAGCACTGCTGGTTCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3088
LEAP-Seq percent confirming:98.4962
LEAP-Seq n confirming:131
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:133

Suggested primers for confirmation by PCR

Suggested primer 1:GGACTGAGGAATTTGGGGCA
Suggested primer 2:CCACAGAGCTCGGAGCATAG