| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048834 |
| Chromosome: | chromosome 8 |
| Location: | 3216130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376740 | (1 of 1) IPR008979//IPR018392 - Galactose-binding domain-like // LysM domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCATTACGGCTAGTGCAGCCGGCAAGTCCGGAGCACTGTGCCGCCAC |
| Internal bar code: | ACTAAGTCAGAGCCTATTAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2612 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTTGAGGGAGAGCTGCAA |
| Suggested primer 2: | GGGTTACAAGAGGGACCTGC |