| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.048847 |
| Chromosome: | chromosome 3 |
| Location: | 4496027 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175750 | (1 of 1) PTHR12461:SF2 - JMJC DOMAIN-CONTAINING PROTEIN 7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGTTGGATGGCCGCTGGGAACTGGAACACGAGGGGAGGACGGTGGGGG |
| Internal bar code: | CGGCCGGCTGGTAGTGACAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 249 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTGCTACCACTCGCAAA |
| Suggested primer 2: | AGTGCTAAGTTGCCCCTGAC |