Insertion junction: CLIP2.048851_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.048851
Chromosome:chromosome 3
Location:5386698
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g183650 GDP4,GDPD4 (1 of 3) K01126 - glycerophosphoryl diester phosphodiesterase (E3.1.4.46, glpQ, ugpQ); Glycerophosphoryl diester phosphodiesterase family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACAGCACGCCCATGATGCGGAGCACAACCCGTGGTGGGCCGATATTGGT
Internal bar code:ACCGGGACATTTAGTCCCTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1703
LEAP-Seq percent confirming:42.4242
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCGTGAAGCGCCATGAG
Suggested primer 2:TCTGGGTCAATTTCCGGCTC