Insertion junction: CLIP2.048863_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048863
Chromosome:chromosome 13
Location:4544614
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g603300 (1 of 781) IPR000104 - Antifreeze protein, type I CDS
lncRNA_TCONS_00191658 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCCGTGTCGGCGGCAGCGCCCGCCTCCGGGTCGCGTGCCTCGCCTGT
Internal bar code:ACCTCTATAGACACTTCAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3249
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTTCAGGAATGGCAACGG
Suggested primer 2:TGGAGGTGGGTCAAAGATGC