| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048866 |
| Chromosome: | chromosome 9 |
| Location: | 5304294 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407373 | (1 of 2) 2.3.1.128 - Ribosomal-protein-alanine N-acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCGCTGCGTGCCGAGGCCGCCGCATCAGTGCTACGGCCGATGAAGCG |
| Internal bar code: | TGGCTCGTGGGTAGATCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 58.3333 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCCCGGGTATTACTCTC |
| Suggested primer 2: | CTTAGAGCTGAGCCAGAGCC |